But I testet GOL-bugfree in EO, using the ZX81x2 rom. And it worked

The problems seems to be my USB driver environment, I use to load the program.
The USB driver updates some sysvars, to return information (the size of the loaded program and an error-code) to BASIC/the program, calling the driver. That is no problem for "normal" BASIC-programs, but probably destroys your very special "Basic" program ...
Sorry for that
Siggi